Septin-microtubule association via a motif unique to the isoform 1 of septin 9 tunes stress fibers.
Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180314 | pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep | bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5 |
| 180319 | pCMV_SEPT2-mApple | mammalian expression of human SEPT2 fused to monomeric Apple |
| 180321 | pCMV_SEPT2 NCmut-mApple | mammalian expression of human SEPT2 NCmut fused to monomeric Apple |
| 180322 | pCMV_SEPT9_i3-msfGFP | mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP |
| 180327 | pCMV_SEPT9_i5-msfGFP | mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP |
| 180328 | pCMV_SEPT9_i1-msfGFP | mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP |
| 180329 | pCMV_SEPT9_i1 NCmut1-msfGFP | mammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFP |
| 180330 | pCMV_SEPT9_i1 NCmut2-msfGFP | mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP |
| 180331 | pCMV_SEPT9_i1 Gmut-msfGFP | mammalian expression of human SEPT9_i1 Gmut fused to monomeric superfolder GFP |
| 180332 | pCMV_SEPT9_i1-i5-msfGFP | mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP |
| 180333 | pCMV_SEPT9_i1 D1-25-msfGFP | mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP |
| 180334 | pCMV_SEPT9_i1 D10-25-msfGFP | mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP |
| 180335 | pCMV_SEPT9_i1 DN7-msfGFP | mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP |
| 180336 | pCMV_SEPT9_i1 R10A R15A-msfGFP | mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP |
| 180337 | pCMV_SEPT9_i1 S12A S13A-msfGFP | mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP |
| 180338 | pCMV_SEPT9_i1 S22A S23A-msfGFP | mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP |
| 180339 | pCMV_SEPT9_i1 R106W-msfGFP | mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP |
| 180340 | pCMV_SEPT9_i1 S111F-msfGFP | mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP |