Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.
Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M
J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180311 | pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP | bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP |
| 180312 | pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6 | bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6 |
| 180313 | pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6 | bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6 |
| 180315 | pCMV_msfGFP-SEPT7 | mammalian expression of human SEPT7 fused to monomeric superfolder GFP |
| 180316 | pCMV_msfGFP-SEPT7 Gmut1 | mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP |
| 180317 | pCMV_mApple-SEPT7 Gmut2 | mammalian expression of human SEPT7 Gmut2 fused to mApple |
| 180318 | pCMV_SEPT2-msfGFP | mammalian expression of human SEPT2 fused to monomeric superfolder GFP |
| 180320 | pCMV_SEPT2 NCmut-msfGFP | mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP |
| 180323 | pCMV_SEPT9_i3-mApple | mammalian expression of human SEPT9_i3 fused to monomeric Apple |
| 180324 | pCMV_SEPT9_i3-mApple-CAAX | mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas) |
| 180325 | pCMV_SEPT9_i3 NCmut-msfGFP | mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP |
| 180326 | pCMV_SEPT9_i3 Gmut-msfGFP | mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP |
| 180341 | pCMV_GFP10-14-SEPT7 | mammalian expression of human SEPT7 fused to GFP10 |
| 180342 | pCMV_GFP11-14-SEPT7 | mammalian expression of human SEPT7 fused to GFP11 |
| 180343 | pCMV_SEPT9_i1-14-GFP10 | mammalian expression of human SEPT9_i1 fused to GFP10 |
| 180344 | pCMV_SEPT9_i1-14-GFP11 | mammalian expression of human SEPT9_i1 fused to GFP11 |
| 180345 | pCMV_SEPT9_i3-14-GFP10 | mammalian expression of human SEPT9_i3 fused to GFP10 |
| 180346 | pCMV_SEPT9_i3-14-GFP11 | mammalian expression of human SEPT9_i3 fused to GFP11 |
| 180347 | pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7 | mammalian co-expression of human SEPT7 fused to GFP10 and of human SEPT7 fused to GFP11 |
| 180348 | pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10 | mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11 |
| 180349 | pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10 | mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11 |
| 180350 | pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10 | mammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11 |
| 180351 | pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10 | mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11 |
| 180352 | pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10 | mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11 |
| 180353 | pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2 | mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11 |
| 180354 | pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1 | mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11 |
| 180355 | pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10 | mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11 |
| 180356 | pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10 | mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11 |
| 180357 | pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10 | mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11 |
| 180358 | pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10 | mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11 |
| 180359 | pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2 | mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11 |
| 180360 | pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10 | mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11 |
| 180361 | pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2 | mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11 |
| 180425 | pCMV_msfGFP-SEPT7 Gmut2 | mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP |