CRISPR activation and interference screens decode stimulation responses in primary human T cells.
Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, Blaeschke F, Ye CJ, Marson A
Science. 2022 Feb 4;375(6580):eabj4008. doi: 10.1126/science.abj4008. Epub 2022 Feb 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180263 | pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64 | Lentiviral expression vector for dCas9-VP64 in mammalian cells |
| 180264 | pZR071_SFFV-dCas9-mCherry-KRAB | Lentiviral expression vector for dCas9-KRAB in mammalian cells |
| 180266 | pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro | Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA |
| 180267 | pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro | Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA |
| 180268 | pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro | Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA |
| 180269 | pZR060_CROPseq-sgCtrl-CRISPRi | Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi |
| 180270 | pZR064_CROPseq-sgCD4-CRISPRi | Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi |
| 180271 | pZR069_CROPseq-sgPTPRC-CRISPRi | Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi |
| 180273 | pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro | Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette |