A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast.
Novarina D, Koutsoumpa A, Milias-Argeitis A
STAR Protoc. 2022 Jun 10;3(2):101358. doi: 10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180282 | pYTK-DN1 | Cas9 cassette (TU1) |
| 180283 | pYTK-DN2 | Spacer cassette (TU3) |
| 180284 | pYTK-DN3 | Spacer cassette (TU2) |
| 180285 | pYTK-DN4 | KanR multi-gene yeast expression vector (GFP-dropout) |
| 180286 | pYTK-DN5 | NatR multi-gene yeast expression vector (GFP-dropout) |
| 180287 | pYTK-DN6 | HygR multi-gene yeast expression vector (GFP-dropout) |