Colicin E1 opens its hinge to plug TolC.
Budiardjo SJ, Stevens JJ, Calkins AL, Ikujuni AP, Wimalasena VK, Firlar E, Case DA, Biteen JS, Kaelber JT, Slusky JSG
Elife. 2022 Feb 24;11. pii: 73297. doi: 10.7554/eLife.73297.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155179 | pTrc99a-NoSS-TolC | Expresses signal sequence-less TolC as inclusion bodies |
| 155180 | pET303-colE1T | Expresses colicin E1 residues 1-190 |
| 155181 | pET303-colE1TR | Expresses colicin E1 residues 1-364 |
| 180233 | pET303-ColE1-T-E192C | Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry |
| 180235 | pET303-ColE1-TR-E192C-del1-40 | Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry |
| 180236 | pET303-ColE1-TR-del1-40 | Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) |
| 180237 | pET303-ColE1-T-P110A | Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110 |