Comparison of NF-kappaB from the protists Capsaspora owczarzaki and Acanthoeca spectabilis reveals extensive evolutionary diversification of this transcription factor.

Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, Gilmore TD
Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
180197	pcDNA-FLAG-Co-NF-kB	Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells
180198	pcDNA-FLAG-Co-RHD	Expresses FLAG-tagged version of the Co-NF-kB Rel Homology Domain
180199	pcDNA-FLAG-Co-NF-kB-Cterm	Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells
180200	pcDNA-FLAG-As-NF-kB1	Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells
180201	pcDNA-FLAG-As-NF-kB2	Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells
180202	pcDNA-FLAG-As-NF-kB3	Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells
180203	pcDNA-MYC-As-NF-kB1	Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells
180204	pcDNA-MYC-As-NF-kB2	Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells
180205	pcDNA-MYC-As-NF-kB3	Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells
180206	pGB-Co-NF-kB	Expression GAL4-fusion of Co-NF-kB in yeast
180207	pGB-Co-RHD	Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast
180208	pGB-Co-Cterm	Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast
180209	pGB-As-NF-kB1	Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast
180210	pGB-As-NF-kB2	Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast
180211	pGB-As-NF-kB3	Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast