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Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities.

Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ
Cell Rep. 2021 May 18;35(7):109126. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
179961pCG_SARS-CoV1 NSP1 C-V5-tagMammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.
179962pCG_SARS-CoV1 NSP3 C-V5-tagMammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.
179963pCG_SARS-CoV1 NSP7 C-V5-tagMammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.
179964pCG_SARS-CoV1 NSP15 C-V5-tagMammalian expression vector for SARS-CoV-1 Nsp15, V5-tagged. Sequence codon-optimized.
179965pCG_SARS-CoV1 NSP16 C-V5-tagMammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.
179966pCG_SARS-CoV1 E C-V5-tagMammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.
179967pCG_SARS-CoV1 M C-V5-tagMammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized.
179968pCG_SARS-CoV1 N C-V5-tagMammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.
179969pCG_SARS-CoV1 C-V5-tag ORF3aMammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.
179970pCG_SARS-CoV1 ORF6 C-V5-tagMammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.
179971pCG_SARS-CoV1 C-V5-tag ORF7aMammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.
179972pCG_SARS-CoV2 NSP1 C-V5-tagMammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized.
179974pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tagMammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence).
179975pCG_SARS-CoV2 NSP15 C-V5-tagMammalian expression vector for SARS-CoV-2 Nsp15, V5-tagged. Sequence codon-optimized.
179976pCG_SARS-CoV2 NSP16 C-V5-tagMammalian expression vector for SARS-CoV-2 Nsp16, V5-tagged. Sequence codon-optimized.
179977pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tagMammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).
179978pCG_SARS-CoV2 M C-V5-tagMammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.
179979pCG_SARS-CoV2 N C-V5-tagMammalian expression vector for SARS-CoV-2 N, V5-tagged. Sequence codon-optimized.
179980pCG_SARS-CoV2 ORF3a C-V5-tagMammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.
179981pCG_SARS-CoV2 ORF7a C-V5-tagMammalian expression vector for SARS-CoV-2 ORF7a, V5-tagged. Sequence codon-optimized.
179982pCG_batCoV RaTG13 NSP1 C-V5-tagMammalian expression vector for RaTG13 Nsp1, V5-tagged. Sequence codon-optimized.
179984pCG_batCoV RaTG13 NSP15 C-V5-tagMammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.
179985pCG_batCoV RaTG13 NSP16 C-V5-tagMammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.
179986pCG_batCoV RaTG13 M C-V5-tagMammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.
179987pCG_batCoV RaTG13 N C-V5-tagMammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.
179988pCG_batCoV RaTG13 ORF3a C-V5-tagMammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.
179989pCG_batCoV RaTG13 ORF7a C-V5-tagMammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized.
179990pCG_SARS-CoV2 ORF8 C-V5-tagMammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.

Antibodies from Article