Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral Infections in Vitro.

Madireddy I, Pierson Smela M
MicroPubl Biol. 2022 Jun 16;2022. doi: 10.17912/micropub.biology.000590. eCollection 2022. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
179913	pX459-sg-mGreenLantern/EGFP	All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.
179914	pX459-sg-mGreenLantern332	All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.
179915	pX459-sg-CMV51	All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.
179916	pX459-sg-CMV152	All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.
179917	pX459-sg-CMV162	All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.
179918	pX459-sg-tdTomato90/816	Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.
179919	pX459-sg-tdTomato166/892	Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.
188482	rtTA-sg-mGreenLantern/EGFP	gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.
188483	rtTA-sg-mGreenLantern332	gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.
188484	rtTA-sg-CMV51	gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.
188485	rtTA-sg-CMV152	gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.
188486	rtTA-sg-CMV162	gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.
188487	rtTA-sg-tdTomato90/816	gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.
188488	rtTA-sg-tdTomato166/892	gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.