On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members.
Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179887 | pCDH-EF1a-HygroR-T2A-TetOn3G | Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector) |
| 179888 | pCDH-EF1a-mTagBFP-T2A-TetOn3G | Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector) |
| 179889 | pCDH-AtAFB2-mTagBFP2 | Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid) |
| 179890 | pJET-07-mNeonGreen-R11a | Donor with homologous arms for Rab11a to knock in mNeonGreen |
| 179891 | pJET-10-mCherry-R11b | Donor with homologous arms for Rab11b to knock in mCherry |
| 179892 | pJET-61-SFFV-T2A-PuroR-polyA-R11a | Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out |
| 179893 | pJET-62-SFFV-T2A-PuroR-polyA-R11b | Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out) |
| 179894 | pJET-63-SFFV-T2A-BlaR-polyA-R11a | Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) |
| 179895 | pJET-64-SFFV-T2A-BlaR-polyA-R11b | Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) |
| 179896 | pMKRQ_11_DExCon-mNeonGreen-R25 | Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module |
| 179897 | pJET-22-DExCon-mNeonGreen-R11a | Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module |
| 179898 | pJET-23-DExCon-mCherry-R11b | Donor with homologous arms for Rab11b to knock in DExCon-mCherry module |
| 179899 | pJET-67-DExCon-mNeonGreen-R11b | Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module |
| 179900 | pJET-59-DExCon-mTagBFP2-R25 | Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module |
| 179901 | pJET-41-DExCon-antiGFPnanobody-mCherry-R11b | Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module |
| 179902 | pJET-42-DExCon-antiGFPnanobody-mCherry-R11a | Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module |
| 179903 | pJET-49-DExogron-mNeonGreen-R11a | Donor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen module |
| 179904 | pJET-50-DExogron-mCherry-R11b | Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module |