Synaptotagmin 7 is targeted to the axonal plasma membrane through gamma-secretase processing to promote synaptic vesicle docking in mouse hippocampal neurons.
Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179713 | pF(UG) hSyn SYT7alpha | Lentiviral expression of mouse Syt7 |
| 179714 | pF(UG) hSyn PP-HaloTag-SYT7alpha | Lentiviral expression of mouse Syt7 with N terminal HaloTag |
| 179715 | pF(UG) hSyn SYT7alpha-HaloTag | Lentiviral expression of mouse Syt7 with C terminal HaloTag |
| 179716 | pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala | Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag |
| 179717 | pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP | Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP |
| 179718 | pF(UG) hSyn SYP-deltaTMD SYT7alpha | Lentiviral expression of synaptic vesicle-targeted mouse Syt7 |
| 179719 | pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag | Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7 |
| 179720 | pF(UG) hSyn PM-SYT7alpha deltaTMD | Lentiviral expression of plasma membrane-targeted mouse Syt7 |
| 179721 | pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag | Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7 |
| 179722 | pF(UG) hSyn Lamp1-SYT7alpha deltaTMD | Lentiviral expression of lysosome-targeted mouse Syt7 |
| 179723 | pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag | Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7 |
| 179724 | pF(UG) U6-SYT7 sgRNA 777 HaloTag | U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI |
| 179725 | pF(UG) hSyn spCas9 | human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI |
| 179726 | pF(UG) CamKII sf iGluSnFR S72A | Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release |
| 179727 | pF(UG) hSyn SYP-mRuby3 | Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker |
| 179728 | pF(UG) hSyn LAMP1-msGFP (JV012) | Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker |
| 179730 | pEF-mRuby3 | Mammalian expression of mRuby3 from the EF1 promoter |