CRISPR-Cas9 bends and twists DNA to read its sequence.
Cofsky JC, Soczek KM, Knott GJ, Nogales E, Doudna JA
Nat Struct Mol Biol. 2022 Apr;29(4):395-402. doi: 10.1038/s41594-022-00756-0. Epub 2022 Apr 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179525 | pJCC_103 SpCas9 T1337C | For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag |
| 179526 | pJCC_162 SpCas9 KES(1107-1109)GG | For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag |
| 179527 | pJCC_163 SpCas9 K233A/K234A/K253A/K263A | For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag |
| 179528 | pJCC_164 SpCas9 R1333A/R1335A | For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag |