Dual-expression system for blue fluorescent protein optimization.

Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia JM, Koster RW, Namikawa K, Piatkevich KD
Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
196115	pTol2-nbt-mTagBFP2-P2A-mScarlet	zebrafish expression of nbt-mTagBFP2-P2A-mScarlet
196114	pTol2-nbt-Electra2-P2A-mScarlet	zebrafish expression of nbt-Electra2-P2A-mScarlet
196113	pTol2-nbt-Electra1-P2A-mScarlet	zebrafish expression of nbt-Electra1-P2A-mScarlet
184943	pAAV-CAG-mTagBFP2-P2A-EGFP	Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells
184942	pActin-Electra2	beta-actin labelling with blue fluorescent protein Electra2
184941	pActin-Electra1	beta-actin labelling with blue fluorescent protein Electra1
184940	pCytERM-mTagBFP2	ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450
184939	pCytERM-EBFP2	ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450
184938	pAAV-CAG-EBFP2-P2A-EGFP	Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells
184937	pAAV-CAG-mBlueberry2-P2A-EGFP	Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells