Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells.
Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H, Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, Kramer A
Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179441 | pDB-hCRY1-mClover3-CD4-bla | Donor vector to knock in mClover3 C-terminal to human CRY1 gene |
| 179442 | pDB-hCRY1-mScarlet-I-CD4-bla | Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene |
| 179444 | pDB-hCRY1-Luciferase-hvTK-bla | Donor vector to knock in firefly Luciferase C-terminal to human CRY1 gene |
| 179445 | pDB-hCRY1-Halo-CD4-bla | Donor vector to knock in Halotag C-terminal to human CRY1 gene |
| 179447 | pDB-hCRY1-SNAP-CD4-bla | Donor vector to knock in SNAPtag C-terminal to human CRY1 gene |
| 179448 | pDB-hPER2-mClover3-CD4-bla | Donor vector to knock in mClover3 N-terminal to human PER2 gene |
| 179449 | pDB-hPER2-mScarlet-I-CD4-bla | Donor vector to knock in mScarlet-I C-terminal to human PER2 gene |
| 179450 | pDB-hPER2-Luciferase-hvTK-bla | Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene |
| 179451 | pDB-hPER2-Halo-CD4-bla | Donor vector to knock in Halotag C-terminal to human PER2 gene |
| 179452 | pDB-hPER2-SNAP-CD4-bla | Donor vector to knock in SNAPtag C-terminal to human PER2 gene |
| 179453 | pLentiCrisprV2 sgRNA hCRY1 c-term | Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in |
| 179454 | pLentiCrisprV2 sgRNA hPER2c-term #1 | Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in |
| 179455 | pLentiCrisprV2 sgRNA hPER2c-term #2 | Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in |
| 179456 | pLentiCrisprV2 sgRNA hPER2c-term #3 | Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in |