Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9.
Sapozhnikov DM, Szyf M
Nat Commun. 2021 Sep 29;12(1):5711. doi: 10.1038/s41467-021-25991-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179390 | pLenti-mCherry-Cre-blast | Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells. |
| 180426 | pLenti-gRNA-puro | Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis. |