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Protease-controlled secretion and display of intercellular signals.

Vlahos AE, Kang J, Aldrete CA, Zhu R, Chong LS, Elowitz MB, Gao XJ
Nat Commun. 2022 Feb 17;13(1):912. doi: 10.1038/s41467-022-28623-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
179633CMVTO-SEAP-26Sfur-3TM-tevs-AAMPSEAP RELEASE construct with AAMP motif control
179634CMVTO-SEAP-26Sfur-3TM-tevs-KKMPTEVP-inducible tri-transmembrane SEAP RELEASE construct
179635CMVTO-SEAP-26Sfur-3TM-hcvs-KKMPHCVP-inducible tri-transmembrane SEAP RELEASE construct
179636CMVTO-SEAP-26Sfur-B2AD-tevs-KKMPTEVP-inducible single TM SEAP RELEASE construct
179637CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP (GS)HCVP-inducible tri-transmembrane SEAP RELEASE with GS linkers
179638CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMPHCVP-inducible single TM SEAP RELEASE construct
179639CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP (GS)HCVP-inducible single TM SEAP RELEASE construct with GS linkers
179640CMVTO-GFP-ECL-3TM-tevs-KKMP V3TEVP-inducible membrane-bound GFP RELEASE construct
179641CMVTO-GFP-ECL-3TM-hcvs-KKMPHCVP-inducible membrane-bound GFP RELEASE construct
179642CMVTO-GFP-26Sfur-3TM-hcvs-KKMPHCVP-inducible tri-transmembrane GFP RELEASE construct
179643CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMPOR Gate (TEVP/HCVP) SEAP RELEASE construct
179644CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMPAND gate (TEVP/HCVP) SEAP RELEASE construct
179645CMVTO-SEAP-26SFur-B2AD-tvmvs-KKMPTVMVP-inducible single TM SEAP RELEASE construct
179646CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMPTVMVP-inducible IL-12 RELEASE construct
179647CMVTO-p450-hcvs-B2AD2-26Sfur-SEAPHCVP-inducible p450 SEAP RELEASE construct
179648CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMPTVMVP-inducible single TM SEAP RELEASE construct with SpyTag
179649CMVTO-g0Non-coding DNA plasmid control
179650CMVTO-H2b-ChemCherry co-transfection marker
179651CMVTO-BFPBFP co-transfection marker
179652CMVTO-HRAS-G12VHRAS active mutant (G12V mutation)
179653CMVTO-KRASKRAS protein
179654CMVTO-KRAS-G12VKRAS active mutant (G12V mutation)
179655CMVTO-KRAS-G12CKRAS active mutant (G12C mutation)
179656CMVTO-KRAS-G12DKRAS active mutant (G12D mutation)
179657CMVTO-TEVPTobacco Etch Viral Protease
179658CMVTO-HCVPHepatitis C Viral Protease
179659CMVTO-TVMVPTobacco Vein Mottling Viral Protease
179660CMVTO-nTVMVP-AP4-tevs-P3-tevs-cTVMVPmutCytosolic TEVP-inducible caged-nTVMVP
179661CMVTO-nTVMVPmut-tevs-AP4-tevs-P3-cTVMVPCytosolic TEVP-inducible caged-cTVMVP
179662CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAXMembrane-bound (CAAX) TEVP-inducible caged-nTVMVP
179663CMVTO-P3-cTVMVPUncaged-cTVMVP with P3 leucine zipper
179664CMVTO-p450-P3-cTVMVPER-associated uncaged-cTVMVP with P3 leucine zipper
179666CMVTO-RBDK65E-nTEVpRas Binding Domain split-nTEVP
179667CMVTO-RBDK65E-cTEVpRas Binding Domain split-cTEVP
179668CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVpMembrane-bound (Fyn) TEVP-inducible caged-cTVMVP
179669CMVTO-p450-nTVMVp-AP4ER-associated uncaged-nTVMVP with AP4 leucine zipper
179670CMVTO-RBDK65E-R89L-nTEVpMutant Ras Binding Domain split-nTEVP
179671CMVTO-RBDK65E-R89L-cTEVpMutant Ras Binding Domain split-cTEVP
179672CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVpMembrane-bound (Lyn) TEVP-inducible caged-cTVMVP
179673CMVTO-p450-nTVMVp-hcvs-AP4HCVP-repressible ER-associated uncaged-nTVMVP
179674CMVTO-p450-Ns4A-tv-HCVp-P3TVMVP-repressible ER-associated HCVP
179675CMVTO-p450-FKBP-nTEVPER-associated FKBP split nTEVP (Rapalog inducible)
179676CMVTO-p450-FRB-cTEVPER-associated FRB split-cTEVP (Rapalog inducible)
179677CMVTO-p450-hcvs-SpyCatcherHCVP-cleavable ER-associated SpyCatcher
179704CMVTO-mCherry-Kir2.1-HA-tevs-RXRTEVP-inducible mCherry-conjugated Kir2.1 RELEASE
179705CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2TEVP-inducible GFP-conjugated Kir2.1 RELEASE
179706CMVTO-gfp-Kir2.1-HA-tevs-KKYLTEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif
179707CMVTO-mCherry-Kir2.1-HA-tvmvs-RXR-V2TVMVP-inducible mCherry-conjugated Kir2.1 RELEASE
179908CMVTO-SEAPSecreted Embryonic Alkaline Phosphatase
179912CMVTO-HA-GFPConstitutively secreted GFP
179920CMVTO-FurinFurin protein
180022CMVTO-Gbp6-TM-CheMembrane-bound anti-GFP nanobody conjugated to mCherry

Antibodies from Article