Augmenting and directing long-range CRISPR-mediated activation in human cells.
Tak YE, Horng JE, Perry NT, Schultz HT, Iyer S, Yao Q, Zou LS, Aryee MJ, Pinello L, Joung JK
Nat Methods. 2021 Sep;18(9):1075-1081. doi: 10.1038/s41592-021-01224-1. Epub 2021 Aug 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136912 | BPK880-pCAG-DmrC-NLS-3xFLAG-VP64 | DmrC with VP64 fused to it's C-terminus; Mammalian expression vector |
| 179296 | BPK1179 | Expresses dCas9 fused to four DmrA domains |
| 179297 | BPK617 | Expresses dCas9 fused to VP64 |
| 179298 | BPK1160 | Expresses dCas9 fused to P65 |
| 179299 | JEH127 | Expresses dCas9 fused to VPR |