Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production.
Schwaiger KN, Voit A, Wiltschi B, Nidetzky B
Microb Cell Fact. 2022 Apr 9;21(1):61. doi: 10.1186/s12934-022-01781-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179272 | CcCDP_p15A | Expresses CcCDP in E. coli BL21(DE3) |
| 179273 | pBICI_high ori | Expresses CuCBP and BaSP in E. coli BL21(DE3) |
| 179274 | pDUBI | Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically |
| 179276 | pPOLY_new | Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3) |