Characterization and computational simulation of human Syx, a RhoGEF implicated in glioblastoma.
Boyd RJ, Olson TL, Zook JD, Stein D, Aceves M, Lin WH, Craciunescu FM, Hansen DT, Anastasiadis PZ, Singharoy A, Fromme P
FASEB J. 2022 Jul;36(7):e22378. doi: 10.1096/fj.202101808RR.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179286 | pHsSyx3-MBP | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6 |
| 179287 | pMBP-HsSyx3 | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site |
| 179288 | pSUMO-HsSyx3 | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site |
| 179289 | pGFP-HsSyx3 | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site |
| 179290 | HIS_TEV_hsSYX3 | E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site |
| 179291 | pMBP-HsSyx-375-792 | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site |
| 179292 | pGST-HsSyx-375-792 | E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site |
| 179486 | KJ740(DE3) | KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression |