Biosensors based on peptide exposure show single molecule conformations in live cells.
Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm JB, Lavis LD, Elston TC, Hahn KM
Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 10.1016/j.cell.2021.09.026. Epub 2021 Oct 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179137 | yPet_cp229-Binder | YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). |
| 179138 | tagSrc(WT)-mCer3 S208F/V224L | mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY). |
| 179139 | Flag-mCherry-Binder | Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY). |
| 179140 | Negative Control Binder | Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide |
| 179141 | tagSrc-HaloTag | The tag is SsrA (AANDENY). Tag is inserted after N534 of Src. |
| 179142 | SnapTag-Binder | Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY). |
| 190439 | binder/tag-WT-FAK-insertionA | The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK. |
| 190440 | binder/tag-WT-FAK-insertionB | The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK. |
| 220998 | Src(WT)-SsrA(Y7I)-mCer3 | This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal |
| 220999 | Src(YF)-SsrA(Y7I)-mCer3 | this is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminal |
| 221000 | Src(KD/YF)-SsrA(Y7I)-mCer3 | this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal |