Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases.
Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179104 | pDY0216 PASTEv1 PE2-P2A-Bxb1 | PASTEv1 PE2-P2A-Bxb1 |
| 179105 | pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT | pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT |
| 179106 | pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT | pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT |
| 179107 | pDY0207 ACTB atgRNA with v1 scaffold | ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA |
| 179108 | pDY0663 ACTB atgRNA with v2 scaffold | ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold |
| 179109 | pDY0209 ACTB Nicking guide +48 | ACTB N-term Nicking guide 1 +48 guide |
| 179110 | pDY0310 TP901-1 Integrase | TP901-1 Integrase expression vector |
| 179111 | pDY0312 PhiBT Integrase | PhiBT Integrase expression vector |
| 179112 | pDY0750 pCAG-NLS-BceINT | BceINT expression vector |
| 179113 | pDY0738 pCAG-NLS-SscINT | SscINT expression vector |
| 179114 | pDY0739 pCAG-NLS-SacINT | SacINT expression vector |
| 179115 | pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle) | Cargo EGFP with AttP Bxb1 site (Parent minicircle) |
| 179116 | pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle) | Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle) |