The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit.
Bean BDM, Whiteway M, Martin VJJ
G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 10.1093/g3journal/jkac154.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178985 | pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance |
| 178986 | pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance |
| 178987 | pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance |
| 178988 | pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR) | Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance |
| 178989 | pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance |
| 178990 | pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR) | Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance |
| 178991 | pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2 |
| 178992 | pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR) | Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2 |
| 178993 | pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3 |
| 178994 | pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR) | Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3 |
| 178995 | pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 178996 | pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 178997 | pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 178998 | pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 178999 | pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 179000 | pBBK16 pCAS-Tyr-[gRNA: 6=ARS416] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 179001 | pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance |
| 179002 | pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179003 | pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179004 | pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179005 | pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179006 | pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179007 | pBBK23 pCAS-Tyr-[gRNA: 6=ARS416] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179008 | pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR) | Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance |
| 179009 | pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes |
| 179010 | pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes |
| 179011 | pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes |
| 179012 | pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes |
| 179013 | pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes |
| 179014 | pBBK30 pHomL-RPL18Bp-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes |
| 179015 | pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes |
| 179016 | pBBK32 pHomL-TEF2p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes |
| 179017 | pBBK33 pHomL-RPL18Bp-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes |
| 179018 | pBBK34 pHomL-TDH3p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes |
| 179019 | pBBK35 pHomL-TEF2p-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes |
| 179020 | pBBK36 pHomL-RPL18Bp-GFPdropout-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal Scarlet(rfp)-tagged genes |
| 179021 | pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes |
| 179022 | pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes |
| 179023 | pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes |
| 179024 | pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes |
| 179025 | pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes |
| 179026 | pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes |
| 179027 | pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with untagged genes |
| 179028 | pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with untagged genes |
| 179029 | pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR) | Parent vector for markerless yeast integration cassettes with untagged genes |
| 179030 | pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus |
| 179031 | pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus |
| 179032 | pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole |
| 179033 | pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules |
| 179034 | pBBK50 pHomL-RPL18Bp-ARC35-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Neon(gfp), a marker for the actin cytoskeleton |
| 179035 | pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum |
| 179036 | pBBK52 pHomL-RPL18Bp-Neon(gfp)-Link-SED5-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SED5, a marker for the early Golgi |
| 179037 | pBBK53 pHomL-RPL18Bp-Neon(gfp)-Link-SFT2-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SFT2, a marker for the late Golgi |
| 179038 | pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane |
| 179039 | pBBK55 pHomL-RPL18Bp-PIL1-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Neon(gfp), a marker for the eisosomes |
| 179040 | pBBK56 pHomL-RPL18Bp-Neon(gfp)-Link-VPS21-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VPS21, a marker for the endosomes |
| 179041 | pBBK57 pHomL-RPL18Bp-SNF7-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Neon(gfp), a marker for the endosome, class E compartment |
| 179042 | pBBK58 pHomL-RPL18Bp-Neon(gfp)-Link-YPT7-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-YPT7, a marker for the vacuole, late endosome |
| 179043 | pBBK59 pHomL-RPL18Bp-Neon(gfp)-Link-VAM3-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VAM3, a marker for the vacuole |
| 179044 | pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole |
| 179045 | pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome |
| 179046 | pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet |
| 179047 | pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet |
| 179048 | pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome |
| 179049 | pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria |
| 179050 | pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria |
| 179051 | pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus |
| 179052 | pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus |
| 179053 | pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole |
| 179054 | pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules |
| 179055 | pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton |
| 179056 | pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum |
| 179057 | pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early Golgi |
| 179058 | pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late Golgi |
| 179059 | pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane |
| 179060 | pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes |
| 179061 | pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomes |
| 179062 | pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartment |
| 179063 | pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome |
| 179064 | pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole |
| 179065 | pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuole |
| 179066 | pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome |
| 179067 | pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet |
| 179068 | pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet |
| 179069 | pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisome |
| 179070 | pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria |
| 179071 | pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondria |
| 179072 | pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane |
| 179073 | pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane |
| 179074 | pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane |
| 179075 | pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum |
| 179076 | pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum |
| 179077 | pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR) | Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol |
| 179078 | pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance |
| 179079 | pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR) | Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance |
| 179080 | pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR) | Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators |