The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase.
van Beljouw SPB, Haagsma AC, Rodriguez-Molina A, van den Berg DF, Vink JNA, Brouns SJJ
Science. 2021 Sep 17;373(6561):1349-1353. doi: 10.1126/science.abk2718. Epub 2021 Aug 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178980 | pTU398 | 13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) |
| 178981 | pTU387 | pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array |
| 178982 | pTU422 | 13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times) |
| 178983 | pTU424 | pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized) |
| 178984 | pTU425 | pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized) |