Boosting plant genome editing with a versatile CRISPR-Combo system.
Pan C, Li G, Malzahn AA, Cheng Y, Leyson B, Sretenovic S, Gurel F, Coleman GD, Qi Y
Nat Plants. 2022 May;8(5):513-525. doi: 10.1038/s41477-022-01151-9. Epub 2022 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178954 | pYPQ-Cas9-Act3.0 | It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation. |
| 178956 | pYPQ-ABE-Cas9n-Act3.0 | It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation. |
| 178957 | pYPQ-SpRY-Act3.0 | It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation. |
| 178958 | pYPQ-CBE-SpRYn-Act3.0 | It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation. |
| 178959 | pYPQ-ABE-SpRYn-Act3.0 | It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation. |
| 179211 | pYPQ132-tRNA | Golden Gate entry vector; 2nd gRNA with tRNA |
| 179212 | pYPQ133-tRNA | Golden Gate entry vector; 3rd gRNA with tRNA |
| 179213 | pYPQ134-tRNA | Golden Gate entry vector; 4th gRNA with tRNA |
| 179216 | pYPQ134B | Golden Gate entry vector; 4th gRNA under AtU3 promoter |