Rapid directed molecular evolution of fluorescent proteins in mammalian cells.
Babakhanova S, Jung EE, Namikawa K, Zhang H, Wang Y, Subach OM, Korzhenevskiy DA, Rakitina TV, Xiao X, Wang W, Shi J, Drobizhev M, Park D, Eisenhard L, Tang H, Koster RW, Subach FV, Boyden ES, Piatkevich KD
Protein Sci. 2022 Mar;31(3):728-751. doi: 10.1002/pro.4261. Epub 2021 Dec 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178965 | pmiRFP2-Actin-C-18 | Expresses the fusion protein of miRFP2-Actin-C in mammalian cells |
| 178966 | pmiRFP2-P2A-HO1-N1 | Expresses miRFP2 and HO1 in mammalian cells |
| 178967 | pCMV-miRFP2-IRES-HO1-N1 | Expresses miRFP2 and HO1 in mammalian cells |
| 178968 | pAAV-CAG-miRFP2 | Expresses miRFP2 in mammalian cells |
| 178969 | pSF11-wmiRFP2 | Expresses miRFP2 in worm cells |
| 178971 | pAAV-CAG-TagRFP658-P2A-GFP | Expresses TagRFP658 and GFP in mammalian cells |
| 178972 | pKeratin-emiRFP2-N1 | Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells |
| 178973 | pphiLOV3-N1 | Expresses phiLOV3 in mammalian cells |
| 178974 | pTagRFP658-N1 | Expresses TagRFP658 in mammalian cells |
| 179485 | pSF11-wmiRFP2-T2A-HO1 | Expresses miRFP2 and HO1in worm cells |
| 196109 | pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3 | mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3 |
| 196110 | pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3 | mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3 |
| 196111 | pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3 | mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3 |
| 196112 | pTol2zmiRFP-EF1-2xCMV-EF1-mClover3 | mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3 |