A detailed protocol for expression, purification, and activity determination of recombinant SaCas9.
Flottmann F, Pohl GM, Gummert J, Milting H, Brodehl A
STAR Protoc. 2022 Apr 5;3(2):101276. doi: 10.1016/j.xpro.2022.101276. eCollection 2022 Jun 17.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178901 | pET28-SaCas9 | SaCas9 (Cas9 from Staphylococcus aureus) |