Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System.
Landry LG, Mann SE, Anderson AM, Nakayama M
Bio Protoc. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883. eCollection 2021 Jan 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153416 | pUS | Backbone for cloning downstream of UCOE-SFFV |
| 153422 | pMIII-Neo | MSCV with IRES-Neomycin resistant gene |
| 162747 | 8xNFAT-ZsG-mCD8 | NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene |
| 162750 | pMSCVII | MSCV backbone |
| 162751 | pMSCVII-AM | Retroviral (MSCV) expression of ametrine fluorescent protein |
| 162752 | pMSCVII-tdTomato | Retroviral (MSCV) expression of td Tomato fluorescent protein |
| 162753 | pMSCVII-mChe | Retroviral (MSCV) expression of mCherry fluorescent protein |
| 162754 | pMSCVII-LO | Retroviral (MSCV) expression of LSSmOrange fluorescent protein |
| 162755 | pMSCVII-BFP | Retroviral (MSCV) expression of mTagBFP2 fluorescent protein |
| 162756 | pMSCVII-E2Crimson | Retroviral (MSCV) expression of E2-Crimson fluorescent protein |