A versatile viral toolkit for functional discovery in the nervous system.
Pouchelon G, Vergara J, McMahon J, Gorissen BL, Lin JD, Vormstein-Schneider D, Niehaus JL, Burbridge TJ, Wester JC, Sherer M, Fernandez-Otero M, Allaway KC, Pelkey K, Chittajallu R, McBain CJ, Fan M, Nasse JS, Wildenberg GA, Fishell G, Dimidschstein J
Cell Rep Methods. 2022 May 26;2(6):100225. doi: 10.1016/j.crmeth.2022.100225. eCollection 2022 Jun 20.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170846 | pAAV-VTKD1 | AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer. |
| 170847 | pAAV-VTKD2 | AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer. |
| 170848 | pAAV-VTKD3 | AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer. |
| 170849 | pAAV-VTKD4 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer. |
| 170850 | pAAV-VTKD5 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in cortical interneurons under the control of the Dlx enhancer. |
| 170851 | pAAV-VTKD6 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer. |
| 170852 | pAAV-VTKS1 | AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter. |
| 170853 | pAAV-VTKS2 | AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter. |
| 170854 | pAAV-VTKS3 | AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter. |
| 170855 | pAAV-VTKS4 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter. |
| 170856 | pAAV-VTKS5 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter. |
| 170857 | pAAV-VTKS6 | AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter. |
| 178696 | pAAV-VTKD2-V5-APEX2-NES | AAV vector for Cre-dependent transgene expression of V5-APEX-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178697 | pAAV-VTKD3-V5-APEX2-NES | AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178698 | pAAV-VTKD4-V5-APEX2-NES | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178699 | pAAV-VTKD5-V5-APEX2-NES | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178700 | pAAV-VTKD6-V5-APEX2-NES | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178702 | pAAV-VTKS2-V5-APEX2-NES | AAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178703 | pAAV-VTKS3-V5-APEX2-NES | AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178704 | pAAV-VTKS4-V5-APEX2-NES | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178706 | pAAV-VTKS1-hChR2-tBFP | AAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178707 | pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP | AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178708 | pAAV-VTKS3-TeTLc-P2A-mCherry | AAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178709 | pAAV-VTKD4-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178710 | pAAV-VTKD5-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178711 | pAAV-VTKD6-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178712 | pAAV-VTKS4-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178713 | pAAV-VTKS5-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178714 | pAAV-VTKS6-GFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178715 | pAAV-VTKD4-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178716 | pAAV-VTKD5-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178717 | pAAV-VTKD6-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178718 | pAAV-VTKS4-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178719 | pAAV-VTKS5-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178720 | pAAV-VTKS6-dTom | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178721 | pAAV-VTKD4-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178722 | pAAV-VTKD5-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178723 | pAAV-VTKD6-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178724 | pAAV-VTKS4-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178725 | pAAV-VTKS5-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178726 | pAAV-VTKS6-ChR2-YFP | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178727 | pAAV-VTKD4-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178728 | pAAV-VTKD5-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178729 | pAAV-VTKD6-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016) |
| 178730 | pAAV-VTKS4-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178731 | pAAV-VTKS5-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |
| 178732 | pAAV-VTKS6-GCaMP6f | AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003) |