Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains.
Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, Mittag T
Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. Epub 2021 Dec 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178658 | WT A1-LCD +NLS | Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320) |
| 178659 | A1-LCD-12F+12Y | Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320) |
| 178660 | A1-LCD+7F-7Y | Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320) |
| 178661 | A1-LCD-9F+6Y | Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, six Tyr added (LCD: low-complexity domain, aa 186-320) |
| 178662 | A1-LCD-8F+4Y | Bacterial expression of N-terminally 6His tagged A1-LCD with eight Phe residues removed, four Tyr added (LCD: low-complexity domain, aa 186-320) |
| 178663 | A1-LCD-9F+3Y | Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320) |
| 178664 | A1-LCD-10R | Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320) |
| 178665 | A1-LCD-6R | Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues removed (LCD: low-complexity domain, aa 186-320) |
| 178666 | A1-LCD+2R | Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues added (LCD: low-complexity domain, aa 186-320) |
| 178667 | A1-LCD+7R | Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added (LCD: low-complexity domain, aa 186-320) |
| 178668 | A1-LCD-2K | Bacterial expression of N-terminally 6His tagged A1-LCD with two Lys residues removed (LCD: low-complexity domain, aa 186-320) |
| 178669 | A1-LCD-3R+3K | Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320) |
| 178670 | A1-LCD-6R+6K | Bacterial expression of N-terminally 6His tagged A1-LCD with six Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320) |
| 178671 | A1-LCD-10R+10K | Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320) |
| 178672 | A1-LCD-4D | Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320) |
| 178673 | A1-LCD+4D | Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues added (LCD: low-complexity domain, aa 186-320) |
| 178674 | A1-LCD+8D | Bacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320) |
| 178675 | A1-LCD+12D | Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Asp residues added (LCD: low-complexity domain, aa 186-320) |
| 178676 | A1-LCD+12E | Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320) |
| 178677 | A1-LCD+7R+10D | Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, ten Glu residues added (LCD: low-complexity domain, aa 186-320) |
| 178678 | A1-LCD+7R+12D | Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320) |
| 178679 | A1-LCD+7K+12D | Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320) |
| 178680 | A1-LCD-2R-2K+3D | Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320) |
| 178681 | A1-LCD-4R-2K+5D | Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320) |
| 178682 | A1-LCD-10G+10S | Bacterial expression of N-terminally 6His tagged A1-LCD with ten Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320) |
| 178683 | A1-LCD-20G+20S | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320) |
| 178684 | A1-LCD-30G+30S | Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320) |
| 178685 | A1-LCD+23G-23S | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320) |
| 178686 | A1-LCD-30G+30S+7F-7Y | Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320) |
| 178687 | A1-LCD-30G+30S-12F+12Y | Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320) |
| 178688 | A1-LCD-20G+20S+7F-7Y | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320) |
| 178689 | A1-LCD-20G+20S-12F+12Y | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320) |
| 178690 | A1-LCD+23G-23S+7F-7Y | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320) |
| 178691 | A1-LCD+23G-23S-12F+12Y | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320) |
| 178692 | A1-LCD-14N+14Q | Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320) |
| 178693 | A1-LCD-23S+23T | Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320) |
| 178694 | A1-LCD-14N-4Q+18G | Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320) |