A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids.
Okamoto T, Natsume Y, Yamanaka H, Fukuda M, Yao R
STAR Protoc. 2021 Sep 16;2(4):100780. doi: 10.1016/j.xpro.2021.100780. eCollection 2021 Dec 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178533 | pCMMP-MCS-IRES-eGFP-P2A-iCas9 | For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells |