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Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids.

Sarrion-Perdigones A, Gonzalez Y, Venken KJT
Methods Mol Biol. 2022;2524:409-432. doi: 10.1007/978-1-0716-2453-1_32. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
178313TB:4xHRE_RE:MiniP4 copies of the HRE DNA binding motif
1783144xHRE_RE:RedFHRE RedFirefly luciferase reporter
1783162xP53_RE::FLucP53 Firefly luciferase reporter
1783176xAP-1_RE::RenillaAP-1 Renilla luciferase reporter
1783183xDBE_RE:NLucFOXO NLuc luciferase reporter
1783197xSMAD_RE::GrRenillaSMAD GreenRenilla luciferase reporter
178320MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβMultiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

Antibodies from Article