Establishment of strigolactone-producing bacterium-yeast consortium.
Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178285 | pCDFDuet-trAtCCD7-OsD27(pYL726 ) | Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis |
| 178286 | pET21a-trAtCCD8(pYL735) | Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis |
| 178287 | pET28b-trAtCCD8(pYL736) | Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis |
| 178288 | p413TEF1-ATR1(pYL573) | Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1 |
| 178289 | p415PGK1-AtMAX1(pYL759) | Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5 |
| 178290 | pAG414GPD-GaCYP722C(pYL777) | Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1 |
| 178291 | pAG414GPD-CaCYP722C(pYL803) | Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1 |
| 178292 | pAG414GPD-LjCYP722C(pYL795) | Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1 |
| 178293 | pAG414GPD-VuCYP722C(pYL1070) | Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1 |
| 178294 | pAG414GPD-SlCYP722C(pYL1071) | Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1 |
| 178295 | pAG414GPD-TpCYP722C(pYL851) | Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1 |
| 178296 | pAG414GPD-CsCYP722C(pYL877) | Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1 |
| 178297 | pAG414GPD-VvCYP722C(pYL852) | Expresses VvCYP722C from V. vinifera in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VvCYP722C-TCYC1 |
| 178298 | pAG414GPD-GmCYP722C(pYL887) | Expresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1 |
| 178299 | pAG414GPD-FvCYP722C2(pYL878) | Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1 |
| 178300 | pAG414GPD-OsCYP711A3(pYL761) | Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1 |
| 178301 | p416PGK1-OsCYP711A2(pYL770) | Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5 |
| 178302 | pAC-BETAipi-ispA/dxs | Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli. |