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Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells.

Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, Covarrubias AA, Sukenik S, Dinneny JR
Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
178189pDRFLIP38-AtLEA4-5SED1 biosensor for expression in Saccharomyces cerevisiae
178190pGPTVII-nlsSED1 SED1 biosensor for expression in Arabidopsis thaliana
178191pDEST-HisMBP-SED1 SED1 biosensor for expression in Escherichia coli
180824pHBS1449 SED1-LentiSED1 biosensor for stable integration and expression in mammalian cells

Antibodies from Article