In vivo inducible reverse genetics in patients' tumors to identify individual therapeutic targets.

Carlet M, Volse K, Vergalli J, Becker M, Herold T, Arner A, Senft D, Jurinovic V, Liu WH, Gao Y, Dill V, Fehse B, Baldus CD, Bastian L, Lenk L, Schewe DM, Bagnoli JW, Vick B, Schmid JP, Wilhelm A, Marschalek R, Jost PJ, Miething C, Riecken K, Schmidt-Supprian M, Binder V, Jeremias I
Nat Commun. 2021 Sep 27;12(1):5655. doi: 10.1038/s41467-021-25963-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
178185	pCDH-SFFV-GLuc-CreERT2-mCherry	the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.