Biochemical reconstitutions reveal principles of human gamma-TuRC assembly and function.
Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, Kapoor TM
J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 10.1083/jcb.202009146.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178067 | pACEBac1-gamma-tubulin-TEV-His6 | Insect cell expression of gamma-tubulin-TEV-His6 |
| 178068 | pACEBac1-GCP2 | Insect cell expression of GCP2 |
| 178069 | pACEBac1-GCP3 | Insect cell expression of GCP3 |
| 178070 | pACEBac1-GCP4 | Insect cell expression of GCP4 |
| 178071 | pACEBac1-GCP5 | Insect cell expression of GCP5 |
| 178072 | pACEBac1-GCP6 | Insect cell expression of GCP6 |
| 178073 | pACEBac1-NEDD1 | Insect cell expression of NEDD1 |
| 178074 | pACEBac1-ZZ-TEV-MZT2-3C-mEGFP | Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP |
| 178075 | pACEBac1-MZT1 | Insect cell expression of MZT1 |
| 178076 | pACEBac1-beta-actin | Insect cell expression of beta-actin |
| 178077 | pACEBac1-gamma-tubulin(N229A)-TEV-His6 | Insect cell expression of gamma-tubulin(N229A)-TEV-His6 |
| 178079 | pACEBac1-gamma-TuSC | Insect cell expression of gamma-TuSC |