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Creation of Bacterial cells with 5-Hydroxytryptophan as a 21(st) Amino Acid Building Block.

Chen Y, Tang J, Wang L, Tian Z, Cardenas A, Fang X, Chatterjee A, Xiao H
Chem. 2020 Oct 8;6(10):2717-2727. doi: 10.1016/j.chempr.2020.07.013. Epub 2020 Aug 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
178042pUltra-5HTPExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
178043pET22b-T5-sfGFP*-XcP4H-MH4RExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

Antibodies from Article