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A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development.

Modzelewski AJ, Shao W, Chen J, Lee A, Qi X, Noon M, Tjokro K, Sales G, Biton A, Anand A, Speed TP, Xuan Z, Wang T, Risso D, He L
Cell. 2021 Oct 7. pii: S0092-8674(21)01104-1. doi: 10.1016/j.cell.2021.09.021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
178028Psicheck Cdk2ap1Exon1expression vector to test translation efficiency by luciferase reporter.
178029psiCheck Cdk2ap1ΔN (MT2B2)expression vector to test translation efficiency by luciferase reporter.
178030pMSCV Cdk2ap1CAN-HARetroviral vector for the purpose of overexpression in mammalian cell culture
178031pMSCV Cdk2ap1ΔN (MT2B2)-HARetroviral vector for the purpose of overexpression in mammalian cell culture
178032pET28a Cdk2ap1CANExpression vector for the purpose of protein purification.
178033pET28a Cdk2ap1CAN-MutTERExpression vector for the purpose of protein purification.
178034pET28a Cdk2ap1ΔN (MT2B2)Expression vector for the purpose of protein purification.
178035pET28a Cdk2ap1ΔN (MT2B2)-MutTERExpression vector for the purpose of protein purification.

Antibodies from Article