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Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used.

Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL
Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
177992pDONR221-DXOFor gateway cloning of DXO
177993pDEST17-DXOFor purification of N-terminally His-tagged DXO from bacteria
177998pcDNA5-FRT/TO-N-mEGFP-PARP11Expresses N-terminally mEGFP-tagged PARP11
178000pcDNA5-FRT/TO-N-mEGFP-PARP15Expresses N-terminally mEGFP-tagged PARP15
178004pcDNA5-FRT/TO-N-mEGFP-PARP7Expresses N-terminally mEGFP-tagged PARP7
178005pcDNA5-FRT/TO-N-mEGFP-PARP6Expresses N-terminally mEGFP-tagged PARP6
184469pDONR221-PARP11For gateway cloning of PARP11
184471pDONR221-PARP15For gateway cloning of PARP15
184474pDONR221-PARP7For gateway cloning of PARP7

Antibodies from Article