Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag.

Dey SK, Filonov GS, Olarerin-George AO, Jackson BT, Finley LWS, Jaffrey SR
Nat Chem Biol. 2021 Dec 22. pii: 10.1038/s41589-021-00925-0. doi: 10.1038/s41589-021-00925-0. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
177913	pAV-U6+27-Squash	Expresses Squash aptamer in mammalian cells using the U6+27 promoter.
177914	pAV-5S-Squash	Expresses 5S rRNA fused to Squash aptamer in mammalian cells
177915	pAV-U6+27-Tornado-Squash	Expresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system.
177916	pAV-U6+27-Tornado-Squash-SAM-sensor 4-2	Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.
177917	pAV-U6+27-Tornado-Squash-SAM-sensor 5-1	Expresses Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.
177918	pAV-U6+27-Tornado-F30-Squash-Broccoli	Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.
177919	pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2	Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.
177920	pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1	Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.