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Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells.

Zhao Y, Harrison DL, Song Y, Ji J, Huang J, Hui E
Cell Rep. 2018 Jul 10;24(2):379-390.e6. doi: 10.1016/j.celrep.2018.06.054. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223569pPPI4-Strep-SNAP-PD-1Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells
223570pPPI4-Strep-SNAP-CD86-His10Protein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cells
223571pHR-CLIP-PD-L1Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells
223572pHR-SNAP-PD-1Lentiviral expression of human PD-1 with SNAP tag in mammalian cells
223573pHR-SNAP-PD-1 I126ALentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells
223574pHR-SNAP-CD86iso2Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells
223576pHR-PD-1-SNAPLentiviral expression of human PD-1 with SNAP tag in mammalian cells
223577pHR-PD-L1-SNAPLentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Antibodies from Article