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PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways.

Zhao Y, Lee CK, Lin CH, Gassen RB, Xu X, Huang Z, Xiao C, Bonorino C, Lu LF, Bui JD, Hui E
Immunity. 2019 Dec 17;51(6):1059-1073.e9. doi: 10.1016/j.immuni.2019.11.003. Epub 2019 Nov 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223578pPPI4-Strep-SNAP-PD-L2-His10Protein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cells
223579pPPI4-Strep-SNAP-CD80-His10Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells
223580pPPI4-Strep-SNAP-CD80 (I92R)-His10Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells
223581pPPI4-Strep-SNAP-CD80Protein purification of human CD80 with Twin-Strep and SNAP tags from mammalian cells
223582pPPI4-Strep-SNAP-CTLA-4-GCN4-His6Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells
223583pPPI4-Strep-SNAP-CTLA-4-His6Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells
223584pPPI4-SNAP-CTLA-4-His6Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells
223585pPPI4-Strep-SNAP-CD28-GCN4-His6Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells
223587pHR-CLIP-PD-L2Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells
223588pHR-SNAP-CD80Lentiviral expression of human CD80 with SNAP tag in mammalian cells
223589pHR-SNAP-CD80 (I92R)Lentiviral expression of human CD80 (I92R) with SNAP tag in mammalian cells
223590pHR-CD80-CLIPLentiviral expression of human CD80 with CLIP tag in mammalian cells
223591pHR-CD80-mGFPLentiviral expression of human CD80 with mGFP tag in mammalian cells
223592pHR-dSV40-CD80-mGFPLentiviral expression of human CD80 with mGFP tag in mammalian cells
223593pHR-CD28-mCherryLentiviral expression of human CD28 with mCherry tag in mammalian cells
223594pHR-dSV40-CTLA-4-mGFPLentiviral expression of human CTLA4 with mGFP tag in mammalian cells
223595pHR-dSV40-CD80-mAppleLentiviral expression of human CD80 with mApple in mammalian cells

Antibodies from Article