PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways.

Zhao Y, Lee CK, Lin CH, Gassen RB, Xu X, Huang Z, Xiao C, Bonorino C, Lu LF, Bui JD, Hui E
Immunity. 2019 Dec 17;51(6):1059-1073.e9. doi: 10.1016/j.immuni.2019.11.003. Epub 2019 Nov 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
223595	pHR-dSV40-CD80-mApple	Lentiviral expression of human CD80 with mApple in mammalian cells
223594	pHR-dSV40-CTLA-4-mGFP	Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells
223593	pHR-CD28-mCherry	Lentiviral expression of human CD28 with mCherry tag in mammalian cells
223592	pHR-dSV40-CD80-mGFP	Lentiviral expression of human CD80 with mGFP tag in mammalian cells
223591	pHR-CD80-mGFP	Lentiviral expression of human CD80 with mGFP tag in mammalian cells
223590	pHR-CD80-CLIP	Lentiviral expression of human CD80 with CLIP tag in mammalian cells
223589	pHR-SNAP-CD80 (I92R)	Lentiviral expression of human CD80 (I92R) with SNAP tag in mammalian cells
223588	pHR-SNAP-CD80	Lentiviral expression of human CD80 with SNAP tag in mammalian cells
223587	pHR-CLIP-PD-L2	Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells
223585	pPPI4-Strep-SNAP-CD28-GCN4-His6	Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells