Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis.

Nagel ZD, Margulies CM, Chaim IA, McRee SK, Mazzucato P, Ahmad A, Abo RP, Butty VL, Forget AL, Samson LD
Proc Natl Acad Sci U S A. 2014 May 6;111(18):E1823-32. doi: 10.1073/pnas.1401182111. Epub 2014 Apr 22. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
177823	pMax_BFP_A191G	Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).
177824	pMax_ScaI_BFP	Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
177825	pMax_GFP	Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)
177826	pMax_GFP_C289T	Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).
177827	pMax_mOrange	Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)
177828	pMax_mOrange_A215C	Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).
177829	pMax_mOrange_G299C	Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).
177830	pMax_mPlum	Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)
177831	pMax_mPlum_C207G/T208C	Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).