Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery.
Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC, Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR
Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177795 | pDEST-DHFR F[1,2]-C (TRP1) | Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays. |
| 177796 | pDEST-DHFR F[3]-C (LEU2) | Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays. |
| 177797 | pDEST-DHFR F[1,2]-N (TRP1) | Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays. |
| 177798 | pDEST-DHFR F[3]-N (LEU2) | Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays. |