ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.
Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177715 | pPLN-hFluc-WT-KDEL | Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention |
| 177716 | pMTS-hFluc | Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting |
| 177717 | pAT-WT-Myc | ER protein alpha-1-antitrypsin (WT), myc tagged |
| 177718 | pATZ-Myc | ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged |
| 177719 | pAT-WT-HA-GFP11 | ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP |
| 177720 | pATZ-HA-GFP11 | ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP |
| 177721 | pATZ-BFP-HA-GFP11 | ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP |
| 177722 | pPLN-hFluc-WT-HA-GFP11-KDEL | Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus |
| 177723 | pPLN-hFluc-DM-HA-GFP11-KDEL | DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus |
| 177724 | pGal-Trans-Myc-HA-GFP11-KDEL | ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP |
| 177725 | pMTS-HA-GFP11 | Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged |
| 177726 | pMTS-hFluc-HA-GFP11 | Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus |
| 177727 | pPLN-hFluc-WT-eGFP-KDEL | Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus |
| 177728 | pPLN-hFluc-DM-eGFP-KDEL | DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus |
| 177729 | pGal-Trans-Myc-eGFP-KDEL | ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged |
| 177730 | p403 – TDH3-MTS-mCherry-GFP1-10-His | mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker |
| 177731 | p406 – TDH3-ER-FlucDM-GFP11-Ura | DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with |
| 177732 | p406 – TDH3-ER-FlucDM-GFP- Ura | DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker |
| 177733 | p406 – TDH3-MTS-GFP11-Ura | Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker |
| 177734 | p406 – TDH3-Elo3-GFP11-Ura | ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker |