Addgene: ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.

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Erik Böttger
Cortes Sanchon et al

ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.

Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
177734	p406 – TDH3-Elo3-GFP11-Ura	ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker
177733	p406 – TDH3-MTS-GFP11-Ura	Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker
177732	p406 – TDH3-ER-FlucDM-GFP- Ura	DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
177731	p406 – TDH3-ER-FlucDM-GFP11-Ura	DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
177730	p403 – TDH3-MTS-mCherry-GFP1-10-His	mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker
177729	pGal-Trans-Myc-eGFP-KDEL	ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged
177728	pPLN-hFluc-DM-eGFP-KDEL	DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
177727	pPLN-hFluc-WT-eGFP-KDEL	Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus
177726	pMTS-hFluc-HA-GFP11	Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus
177725	pMTS-HA-GFP11	Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

Antibodies from Article