A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii.
Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 10.1093/synbio/ysab031. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177696 | pDIV488 | Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species |
| 177697 | pDIV489 | Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species. |
| 177698 | pDIV490 | Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species. |
| 177699 | pDIV491 | Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species. |
| 177700 | pDIV492 | Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species. |
| 177701 | pDIV494 | Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii |
| 177702 | pDIV495 | Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii |
| 177703 | pDIV515 | Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii |
| 177704 | pDIV537 | Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii |