Expanding the targeting scope of FokI-dCas nuclease systems with SpRY and Mb2Cas12a.
Cheng Y, Sretenovic S, Zhang Y, Pan C, Huang J, Qi Y
Biotechnol J. 2022 Apr 4:e2100571. doi: 10.1002/biot.202100571.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
177681 | pYPQ-NF1-L1-dSpRY | Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing |
177682 | pYPQ-NF1-L2-dSpRY | Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing |
177683 | pYPQ-NF-L1-dMb2Cas12a | Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing |
177684 | pYPQ-NF-L2-dMb2Cas12a | Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing |
177686 | pYPQ-NF-L3-NF-L1-dMb2Cas12a | Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing |