Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas.
Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ
Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177628 | pSIBR001 | SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a |
| 177665 | pSIBR002 | SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a |
| 177666 | pSIBR003 | SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a |
| 177667 | pSIBR004 | SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a |
| 177668 | pSIBR005 | Constitutive expression of FnCas12a |