Engineering of AAV-Mediated in Vivo Targeted DNA Methylation Editing System via Staphylococcus Aureus Derived Cas9-SunTag
Yamamoto N, Morita S, Hatada I, Ideta-Otsuka M, Tamura H, Narita M, Igarashi K
Research Square [Preprint] 2021. doi: 10.21203/rs.3.rs-1065603/v1.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177342 | AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA | AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence |
| 177344 | AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA | AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter |
| 177345 | AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA | AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter |
| 177347 | AAV-CMV-scFVDnmt3AL_bGHpA | AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing |
| 177348 | AAV-hSyn-scFVDnmt3AL_bGHpA | AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editing |
| 177349 | AAV-CMV-scFVDnmt3A_bGHpA | AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing |
| 177350 | AAV-CMV-scFVDnmt3L_bGHpA | AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing |
| 177351 | AAV-CMV-scFVtet1_bGHpA | AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing |
| 177352 | AAV-CMV-scFVtet2_bGHpA | AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing |
| 177353 | AAV-hSyn-scFVtet2_bGHpA | AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing |
| 177354 | AAV-TRE-scFVDnmt3AL_bGHpA | AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing |
| 177355 | AAV-TRE-scFVDnmt3A_bGHpA | AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing |
| 177356 | AAV-TRE-scFVDnmt3L_bGHpA | AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing |
| 177357 | AAV-TRE-scFVtet1_bGHpA | AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing |
| 177358 | AAV-TRE-scFVtet2_bGHpA | AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing |
| 177360 | AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA | AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter |
| 177365 | AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA | AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression |