Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems.
Tickman BI, Burbano DA, Chavali VP, Kiattisewee C, Fontana J, Khakimzhan A, Noireaux V, Zalatan JG, Carothers JM
Cell Syst. 2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 10.1016/j.cels.2021.10.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177322 | pCD017 | dCas9 expression |
| 177323 | pBT005 | MCP-SoxS (R93A) expression |
| 177324 | pBT009.J3.RFP | Activatable RFP expression |
| 177325 | pBT009.J1.J306 | scRNA targeting J3 promoter for activation |
| 177326 | pBT009.J3.RR2 | sgRNA targeting RFP for repression |
| 177327 | pCK389.AAV | aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression |