Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12.
Khan OM, Almagro J, Nelson JK, Horswell S, Encheva V, Keyan KS, Clurman BE, Snijders AP, Behrens A
Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197452 | pDEST-Myc-FBW7 Delta F-Box | The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting. |
| 197453 | pDEST-Myc-FBW7 Delta D-Domain | The plasmid expresses D-domain deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression and detection by western blotting. |
| 197454 | pDEST-Myc-FBW7 Delta WD40 (-2 to 7) | The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting. |
| 197455 | pDEST-Myc-FBW7 WD40 (All deleted) | The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting. |
| 197456 | pDEST-GST-FBW7 alpha | The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha. |
| 200715 | pDEST-Myc-FBW7]K404R | The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting. |
| 200716 | pDEST-Myc-FBW7K404/412/R | The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting. |
| 200717 | pDEST-Flag-FBW7K404R | The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting. |