Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12.

Khan OM, Almagro J, Nelson JK, Horswell S, Encheva V, Keyan KS, Clurman BE, Snijders AP, Behrens A
Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5. PubMed Article

Plasmids from Article

Showing 1 to 8 of 8 entries

Show 102550100All entries

Search table:

• Previous
• 1
• Next

ID	Plasmid	Purpose
200717	pDEST-Flag-FBW7K404R	The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.
200716	pDEST-Myc-FBW7K404/412/R	The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.
200715	pDEST-Myc-FBW7]K404R	The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.
197456	pDEST-GST-FBW7 alpha	The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.
197455	pDEST-Myc-FBW7 WD40 (All deleted)	The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.
197454	pDEST-Myc-FBW7 Delta WD40 (-2 to 7)	The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
197453	pDEST-Myc-FBW7 Delta D-Domain	The plasmid expresses D-domain deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression and detection by western blotting.
197452	pDEST-Myc-FBW7 Delta F-Box	The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.