Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson G, Schulte G
ACS Pharmacol Transl Sci. 2021 May 11;4(3):1235-1245. doi: 10.1021/acsptsci.1c00084. eCollection 2021 Jun 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195839 | HiBiT-FZD1 | Expresses human FZD1 with an N-terminal HiBiT |
| 195840 | HiBiT-FZD2 | Expresses human FZD2 with an N-terminal HiBiT |
| 195841 | HiBiT-FZD3 | Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I |
| 195842 | HiBiT-FZD4 | Expresses human FZD4 with an N-terminal HiBiT |
| 195843 | HiBiT-FZD5 | Expresses human FZD5 with an N-terminal HiBiT |
| 195844 | HiBiT-FZD6 | Expresses human FZD6 with an N-terminal HiBiT; contains M345L |
| 195845 | HiBiT-FZD7 | Expresses human FZD7 with an N-terminal HiBiT |
| 195846 | HiBiT-FZD8 | Expresses human FZD8 with an N-terminal HiBiT |
| 195847 | HiBiT-FZD9 | Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H |
| 195848 | HiBiT-FZD10 | Expresses human FZD10 with an N-terminal HiBiT |